Properties of Bromphenol Blue as an Electron Donor for Higher Plant NADH: Nitrate Reductase
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منابع مشابه
Properties of Bromphenol Blue as an Electron Donor for Higher Plant NADH: Nitrate Reductase.
Bromphenol blue, which was reduced with dithionite, was found to support nitrate reduction catalyzed by squash NADH:nitrate reductase at a rate about 5 times greater than NADH with freshly prepared enzyme and 10 times or more with enzyme having been frozen and thawed. Kinetic analysis of bromphenol blue as a substrate for squash nitrate reductase yielded apparent K(m) values of 60 micromolar fo...
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A NADH-nitrate reductase inhibitor has been isolated from young soybean (Glycine max L. Merr. Var. Amsoy) leaves that had been in the dark for 54 hours. The presence of the inhibitor was first suggested by the absence of nitrate reductase activity in the homogenate until the inhibitor was removed by diethylaminoethyl (DEAE)-cellulose chromatography. The inhibitor inactivated the enzyme in homog...
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The search for suitable staining methods for use in electron microscopy has led to many and diverse attempts to increase the opacity to electrons of different structures in tissue sections. The most obvious approach has been the attempt to bind heavy metals to specific sites, either to increase the general contrast or to identify the chemical nature of the structures so stained. Other than the ...
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A barley (Hordeum vulgare L.) mutant, nar1a (formerly Az12), deficient in NADH nitrate reductase activity is, nevertheless, capable of growth with nitrate as the sole nitrogen source. In an attempt to identify the mechanism(s) of nitrate reduction in the mutant, nitrate reductase from nar1a was characterized to determine whether the residual activity is due to a leaky mutation or to the presenc...
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The assimilatory nitrate reductase from Chlorella contains flavin, heme, and molybdenum as prosthetic groups. The molybdenum in assimilatory nitrate reductase is associated with a pterin moiety (molybdopterin) as evidenced by the ability of the enzyme to donate active molybdenum cofactor to the Neurospora nitrate reductase mutant nit-1 and by the oxidative conversion of the pterin to two well c...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1986
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.82.3.729